1 00:00:04,309 --> 00:00:01,670 frequently the animals groom their 2 00:00:06,869 --> 00:00:04,319 wounds and for what period of time and 3 00:00:10,390 --> 00:00:06,879 then total grooming overall as well the 4 00:00:12,470 --> 00:00:10,400 frequency of that and the duration 5 00:00:14,709 --> 00:00:12,480 this is lisa if you don't know what a 6 00:00:16,870 --> 00:00:14,719 bush baby looks like 7 00:00:19,590 --> 00:00:16,880 lisa was in our study she ended up being 8 00:00:21,830 --> 00:00:19,600 in the prayer group and i have a picture 9 00:00:24,470 --> 00:00:21,840 here of a wound you can see on her left 10 00:00:27,109 --> 00:00:24,480 paw it was a fairly minor wound some of 11 00:00:29,109 --> 00:00:27,119 them can really get um quite large but 12 00:00:30,470 --> 00:00:29,119 you can see the fur is lost and the skin 13 00:00:34,229 --> 00:00:30,480 is abraded 14 00:00:38,790 --> 00:00:36,150 measures were assessed at baseline and 15 00:00:41,030 --> 00:00:38,800 end of study blood samples were used for 16 00:00:42,709 --> 00:00:41,040 the physiological measures 17 00:00:45,910 --> 00:00:42,719 all blood samples were taken in the 18 00:00:49,190 --> 00:00:45,920 morning between 7 and 9 30 a.m 19 00:00:50,950 --> 00:00:49,200 cortisol measures we had to get got 20 00:00:52,709 --> 00:00:50,960 we had to have gotten the cortisol 21 00:00:55,110 --> 00:00:52,719 within three minutes of the animal 22 00:00:57,270 --> 00:00:55,120 capture to control for artificial 23 00:00:59,590 --> 00:00:57,280 inflation of cortisol secondary to the 24 00:01:02,470 --> 00:00:59,600 stress of being captured in their cage 25 00:01:04,469 --> 00:01:02,480 and being brought into the lab 26 00:01:06,550 --> 00:01:04,479 i stratified the wounds by wound 27 00:01:09,109 --> 00:01:06,560 severity so we'll be looking at the more 28 00:01:12,230 --> 00:01:09,119 minor wounds mild to moderate and wounds 29 00:01:14,149 --> 00:01:12,240 that were more severe of moderate to 30 00:01:16,310 --> 00:01:14,159 severe severity 31 00:01:18,550 --> 00:01:16,320 procedure animal the trial was double 32 00:01:20,469 --> 00:01:18,560 blind it was someone uninvolved with the 33 00:01:22,390 --> 00:01:20,479 study that randomized the animals dealt 34 00:01:23,910 --> 00:01:22,400 with the intercessors 35 00:01:25,910 --> 00:01:23,920 all animals were treated for 36 00:01:28,230 --> 00:01:25,920 self-injurious behavior so they all 37 00:01:30,070 --> 00:01:28,240 received oral l-tryptophan to try to 38 00:01:31,910 --> 00:01:30,080 help with their condition 39 00:01:34,789 --> 00:01:31,920 there were two groups the animals who 40 00:01:38,069 --> 00:01:34,799 were receiving prayer and tryptophan and 41 00:01:40,069 --> 00:01:38,079 our usual care or tryptophan only group 42 00:01:41,270 --> 00:01:40,079 they received the tryptophan daily 43 00:01:44,390 --> 00:01:41,280 crushed in 44 00:01:46,789 --> 00:01:44,400 yogurt and fed before their meal time 45 00:01:48,950 --> 00:01:46,799 the ip was coincident with the first day 46 00:01:51,749 --> 00:01:48,960 of tryptophan administration and the 47 00:01:53,990 --> 00:01:51,759 prayer continued for four weeks 48 00:01:56,389 --> 00:01:54,000 the intercessors were self-identified as 49 00:01:57,910 --> 00:01:56,399 christian they all engaged in a regular 50 00:01:59,830 --> 00:01:57,920 prayer practice 51 00:02:01,830 --> 00:01:59,840 they all had prior experience with 52 00:02:03,910 --> 00:02:01,840 praying for healing from a distance it 53 00:02:06,870 --> 00:02:03,920 was important to me to try to capture as 54 00:02:09,830 --> 00:02:06,880 many intercessors who had prior clinical 55 00:02:11,990 --> 00:02:09,840 trial prayer experience and most of the 56 00:02:14,229 --> 00:02:12,000 intercessors in fact did 57 00:02:16,710 --> 00:02:14,239 all held the belief that god wants all 58 00:02:18,470 --> 00:02:16,720 creatures healthy and whole it was i 59 00:02:20,550 --> 00:02:18,480 learned a great deal from this study in 60 00:02:22,229 --> 00:02:20,560 many levels and one was 61 00:02:24,550 --> 00:02:22,239 the number of christian intercessors who 62 00:02:26,309 --> 00:02:24,560 would not pray for animals uh believing 63 00:02:29,430 --> 00:02:26,319 that god would only want people to be 64 00:02:31,910 --> 00:02:29,440 healthy and whole so um i i 65 00:02:33,670 --> 00:02:31,920 i found them there are those of us who 66 00:02:35,910 --> 00:02:33,680 uh hold that belief 67 00:02:38,550 --> 00:02:35,920 and prayer was provided daily during the 68 00:02:40,470 --> 00:02:38,560 four-week time frame 69 00:02:42,390 --> 00:02:40,480 the intercessors only received the 70 00:02:44,550 --> 00:02:42,400 animal's names so they would have 71 00:02:47,670 --> 00:02:44,560 received a list that was lisa shawn 72 00:02:49,030 --> 00:02:47,680 daryl felix and so on that's all they 73 00:02:50,710 --> 00:02:49,040 received there were intercessors who 74 00:02:51,830 --> 00:02:50,720 didn't even know what a bush baby looked 75 00:02:53,830 --> 00:02:51,840 like 76 00:02:55,910 --> 00:02:53,840 they were asked to pray daily within the 77 00:02:57,670 --> 00:02:55,920 specific study dates i provided them 78 00:02:59,270 --> 00:02:57,680 with no feedback in terms of the 79 00:03:01,190 --> 00:02:59,280 animal's progress 80 00:03:03,670 --> 00:03:01,200 they were told what the bush babies 81 00:03:06,229 --> 00:03:03,680 looked like so that they could have 82 00:03:08,390 --> 00:03:06,239 an image in their mind 83 00:03:11,430 --> 00:03:08,400 and desired outcomes i opted for 84 00:03:12,550 --> 00:03:11,440 directed prayer but did not control to 85 00:03:14,149 --> 00:03:12,560 check to see whether or not the 86 00:03:16,070 --> 00:03:14,159 intercessors actually followed the 87 00:03:18,470 --> 00:03:16,080 specific direction so they were asked to 88 00:03:20,390 --> 00:03:18,480 pray for effectiveness of the medication 89 00:03:23,750 --> 00:03:20,400 that the behaviors become more calm and 90 00:03:27,350 --> 00:03:23,760 non-stressed and that the wounds healed 91 00:03:29,830 --> 00:03:27,360 um took a look by ancova at wound immune 92 00:03:32,149 --> 00:03:29,840 and cortisol variables controlling for 93 00:03:34,390 --> 00:03:32,159 age because of the changes in the immune 94 00:03:37,030 --> 00:03:34,400 system as one ages it becomes less 95 00:03:39,350 --> 00:03:37,040 effective and mancoba to take a look at 96 00:03:42,470 --> 00:03:39,360 the red blood cell variables and 97 00:03:44,949 --> 00:03:42,480 behavioral variables by t-test here's a 98 00:03:47,750 --> 00:03:44,959 snapshot of the ip or the intercessory 99 00:03:52,309 --> 00:03:47,760 prayer in the non-prayer group 100 00:03:55,430 --> 00:03:52,319 by gender age was comparable as well 101 00:03:56,390 --> 00:03:55,440 and let's move into some of the goodies 102 00:04:00,949 --> 00:03:56,400 here 103 00:04:02,789 --> 00:04:00,959 like i have this divided up in three 104 00:04:04,070 --> 00:04:02,799 ways so you'll see this is for both 105 00:04:07,750 --> 00:04:04,080 groups intercessory prayer 106 00:04:10,309 --> 00:04:07,760 non-intercessory prayer ip1 and nip1 are 107 00:04:13,670 --> 00:04:10,319 the mild to moderate wounds and when you 108 00:04:16,789 --> 00:04:13,680 see ip2 and nip2 those are the more 109 00:04:18,629 --> 00:04:16,799 significant wounds that the animals had 110 00:04:21,030 --> 00:04:18,639 there was no significant difference at 111 00:04:23,110 --> 00:04:21,040 baseline we can see that in fact the 112 00:04:25,189 --> 00:04:23,120 wounds are relatively comparable across 113 00:04:27,430 --> 00:04:25,199 both groups although there was a fairly 114 00:04:28,790 --> 00:04:27,440 significant standard deviation that was 115 00:04:31,189 --> 00:04:28,800 accounted for 116 00:04:32,870 --> 00:04:31,199 by the animals that were down here the 117 00:04:35,189 --> 00:04:32,880 wounds were quite different in the mild 118 00:04:38,150 --> 00:04:35,199 to moderate group much smaller 119 00:04:40,390 --> 00:04:38,160 much less significant 120 00:04:43,030 --> 00:04:40,400 and here's a picture of the wound area 121 00:04:44,870 --> 00:04:43,040 at baseline so that we see the prayer 122 00:04:46,469 --> 00:04:44,880 groups are on the left 123 00:04:48,550 --> 00:04:46,479 no prayer are going to be sitting on the 124 00:04:50,150 --> 00:04:48,560 right on the graphs that i have and 125 00:04:52,390 --> 00:04:50,160 there's about three quarters of a 126 00:04:54,070 --> 00:04:52,400 centimeter difference in the mild to 127 00:04:57,110 --> 00:04:54,080 moderate animals 128 00:04:59,909 --> 00:04:57,120 here we've got maybe a centimeter and a 129 00:05:03,270 --> 00:04:59,919 half that's uh different between those 130 00:05:06,710 --> 00:05:03,280 two groups at baseline 131 00:05:09,270 --> 00:05:06,720 the only significant variable 132 00:05:10,950 --> 00:05:09,280 no differences in behavior wound area 133 00:05:12,950 --> 00:05:10,960 and so on the only significant 134 00:05:14,230 --> 00:05:12,960 physiological variable had to do with 135 00:05:16,790 --> 00:05:14,240 monocytes 136 00:05:21,510 --> 00:05:16,800 and they were significantly lower in the 137 00:05:25,749 --> 00:05:23,670 end of study so what happened after four 138 00:05:27,990 --> 00:05:25,759 weeks of praying to the wounds i've 139 00:05:30,469 --> 00:05:28,000 included the baseline data in orange 140 00:05:32,629 --> 00:05:30,479 here so we can see if we take a look at 141 00:05:34,469 --> 00:05:32,639 the ip group off the bat it dropped from 142 00:05:37,110 --> 00:05:34,479 a little over 10 centimeters to not 143 00:05:38,870 --> 00:05:37,120 quite two with a standard deviation 144 00:05:41,430 --> 00:05:38,880 that's markedly different from where we 145 00:05:43,350 --> 00:05:41,440 were at baseline we actually see in the 146 00:05:46,150 --> 00:05:43,360 prayer that would the group that did not 147 00:05:48,310 --> 00:05:46,160 receive prayer that although the overall 148 00:05:49,590 --> 00:05:48,320 size of the um 149 00:05:51,749 --> 00:05:49,600 well the wounds and the standard 150 00:05:53,990 --> 00:05:51,759 deviation went down somewhat thank you 151 00:05:56,710 --> 00:05:54,000 i'll move through this fairly quickly 152 00:06:00,230 --> 00:05:56,720 i'm down to five minutes here so what we 153 00:06:02,150 --> 00:06:00,240 see happening is that the biggest change 154 00:06:04,550 --> 00:06:02,160 is in the animals with the more severe 155 00:06:06,309 --> 00:06:04,560 wounds where we had three quarters of a 156 00:06:08,070 --> 00:06:06,319 centimeter difference there we're down 157 00:06:10,550 --> 00:06:08,080 to about one and a half 158 00:06:13,110 --> 00:06:10,560 but here we're down to not quite three 159 00:06:14,629 --> 00:06:13,120 centimeters down from 20 centimeters and 160 00:06:15,430 --> 00:06:14,639 change 161 00:06:17,430 --> 00:06:15,440 so 162 00:06:19,110 --> 00:06:17,440 um a significant difference i'm going to 163 00:06:21,110 --> 00:06:19,120 move through relatively quickly so that 164 00:06:24,309 --> 00:06:21,120 you can see all the graphs a significant 165 00:06:26,309 --> 00:06:24,319 difference in the end of wound area 166 00:06:29,670 --> 00:06:26,319 here's what happened remembering the 167 00:06:30,870 --> 00:06:29,680 graphs at baseline we see that this is 168 00:06:33,350 --> 00:06:30,880 changed 169 00:06:35,670 --> 00:06:33,360 decreased 170 00:06:37,350 --> 00:06:35,680 in the less severe group and markedly in 171 00:06:39,189 --> 00:06:37,360 the more severe group 172 00:06:41,029 --> 00:06:39,199 we also had significant differences in 173 00:06:43,270 --> 00:06:41,039 terms of red blood cell 174 00:06:45,350 --> 00:06:43,280 data everything was significant what 175 00:06:47,270 --> 00:06:45,360 happened in red blood cells increased in 176 00:06:49,670 --> 00:06:47,280 the prayed for group they decreased in 177 00:06:52,150 --> 00:06:49,680 the group that did not receive prayer 178 00:06:53,670 --> 00:06:52,160 we saw hemoglobin increased in the group 179 00:06:56,469 --> 00:06:53,680 that was prayed for 180 00:06:59,029 --> 00:06:56,479 slight increase in the no prayer group 181 00:07:00,790 --> 00:06:59,039 hematocrit also increased in the prayer 182 00:07:03,430 --> 00:07:00,800 group less so in the group with no 183 00:07:06,070 --> 00:07:03,440 prayer what happened to the blood cells 184 00:07:07,990 --> 00:07:06,080 themselves again significant differences 185 00:07:11,110 --> 00:07:08,000 other than the concentration was 186 00:07:13,510 --> 00:07:11,120 approaching significance the mchc the 187 00:07:15,830 --> 00:07:13,520 other variables were significant 188 00:07:17,830 --> 00:07:15,840 interestingly the size of the corpuscle 189 00:07:20,550 --> 00:07:17,840 decreased in the group that was prayed 190 00:07:22,870 --> 00:07:20,560 for slightly where it increased in the 191 00:07:25,029 --> 00:07:22,880 group that was not prayed for so we have 192 00:07:27,670 --> 00:07:25,039 smaller red blood cells there was a 193 00:07:29,909 --> 00:07:27,680 consequent change in terms of the amount 194 00:07:32,469 --> 00:07:29,919 of hemoglobin concentration with an 195 00:07:34,870 --> 00:07:32,479 increase in the prayer group 196 00:07:37,029 --> 00:07:34,880 the monocyte levels this was the only 197 00:07:39,189 --> 00:07:37,039 immune data that i had that changed 198 00:07:41,990 --> 00:07:39,199 remembering that the more severe animals 199 00:07:44,550 --> 00:07:42,000 at baseline were significantly lower 200 00:07:45,990 --> 00:07:44,560 they changed to become significantly 201 00:07:49,029 --> 00:07:46,000 greater here's what happened to the 202 00:07:50,790 --> 00:07:49,039 monocytes in that group this was 203 00:07:56,550 --> 00:07:50,800 i'm sorry i've got my 204 00:08:00,950 --> 00:07:58,309 here's where they were at baseline 205 00:08:02,869 --> 00:08:00,960 that's what happened at end of study 206 00:08:05,430 --> 00:08:02,879 so the animals that needed it the most 207 00:08:07,350 --> 00:08:05,440 got it where they needed it cortisol was 208 00:08:09,189 --> 00:08:07,360 not statistically significant but i 209 00:08:11,909 --> 00:08:09,199 present this data because it was a power 210 00:08:13,909 --> 00:08:11,919 issue clearly cortisol changed more in 211 00:08:16,550 --> 00:08:13,919 the direction we expected in the prayer 212 00:08:17,350 --> 00:08:16,560 for the prayer group than the non-prayer 213 00:08:18,710 --> 00:08:17,360 group 214 00:08:21,350 --> 00:08:18,720 although there was a decrease in 215 00:08:23,749 --> 00:08:21,360 cortisol in the animals that weren't uh 216 00:08:25,350 --> 00:08:23,759 prayed for what happened to grooming 217 00:08:28,150 --> 00:08:25,360 changes in the direction that we would 218 00:08:29,909 --> 00:08:28,160 expect the duration not the frequency of 219 00:08:31,670 --> 00:08:29,919 the number of times that the animals 220 00:08:33,110 --> 00:08:31,680 grew but the amount of time that they 221 00:08:35,430 --> 00:08:33,120 spent grooming their wounds was 222 00:08:38,230 --> 00:08:35,440 decreased significantly and the amount 223 00:08:40,709 --> 00:08:38,240 of time they spent overall grooming was 224 00:08:42,230 --> 00:08:40,719 significantly decreased and here's what 225 00:08:45,430 --> 00:08:42,240 those graphs 226 00:08:50,230 --> 00:08:48,070 so in summary the study supports ip 227 00:08:52,310 --> 00:08:50,240 induced improvements in wound healing 228 00:08:54,949 --> 00:08:52,320 the ip animals had greater reduction in 229 00:08:56,550 --> 00:08:54,959 wound area than the non-ip animals one 230 00:08:58,470 --> 00:08:56,560 of the strengths of the study is the 231 00:09:01,269 --> 00:08:58,480 convergent data that suggests that there 232 00:09:03,190 --> 00:09:01,279 are in fact pathways that would lead to 233 00:09:05,829 --> 00:09:03,200 the improved wound healing on the part 234 00:09:08,389 --> 00:09:05,839 of the animals to make some sense of the 235 00:09:10,070 --> 00:09:08,399 blood data when we see increased numbers 236 00:09:12,870 --> 00:09:10,080 of red blood cells hemoglobin and 237 00:09:15,670 --> 00:09:12,880 hematocrit that would support increased 238 00:09:17,590 --> 00:09:15,680 oxygenation which is critical to improve 239 00:09:20,389 --> 00:09:17,600 healing across time 240 00:09:22,790 --> 00:09:20,399 reduction in mean corpuscular volume 241 00:09:25,509 --> 00:09:22,800 hypothesis but perhaps that assisted 242 00:09:27,990 --> 00:09:25,519 with the extraction capability of being 243 00:09:30,070 --> 00:09:28,000 able to remove oxygen at the wound site 244 00:09:31,670 --> 00:09:30,080 by having somewhat smaller red blood 245 00:09:33,750 --> 00:09:31,680 cells 246 00:09:35,269 --> 00:09:33,760 in terms of the immunological changes 247 00:09:37,670 --> 00:09:35,279 there's some debate whether or not 248 00:09:40,070 --> 00:09:37,680 monocytes or macrophages once they live 249 00:09:41,350 --> 00:09:40,080 in tissue are important to wound healing 250 00:09:42,870 --> 00:09:41,360 there's some question in the animal 251 00:09:45,190 --> 00:09:42,880 literature but we'll air on the side 252 00:09:47,030 --> 00:09:45,200 that in fact they are the significant 253 00:09:49,190 --> 00:09:47,040 improvement in monocytes may have 254 00:09:50,790 --> 00:09:49,200 actually supported the dramatic increase 255 00:09:52,949 --> 00:09:50,800 seen in the more severe group that 256 00:09:55,750 --> 00:09:52,959 received prayer 257 00:09:57,750 --> 00:09:55,760 changes in grooming and a decrease in